Illumina sequencing

Illumina sequencing

The Illumina next-generation sequencing (NGS) method is based on sequencing-by-synthesis (SBS), and reversible dye-terminators that enable the identification of single bases as they are introduced into DNA strands. Illumina has been adopted as the key platform in clinical and research NGS applications including, somatic, germline, transcriptome, as well as DNA modification assay such as methylated DNA.

Both Illumina and Ion Torrent provide alternative methods for researchers to study DNA and RNA at the sequence level. There is a high concordance between both technologies in terms of gene-level read counts and sequences, which are in agreement demonstrated by comparison studies. They have been adopted by leading cancer research institutions around the world and have been used to profile thousands of samples in different translational and clinical research projects including the NCI MATCH trial, delivering consistently reliable results.